Supplementary figure 1 mirna screening a nd correlation analysis with reduced bone formation in human and mice. We show that mir 214 participates in the inhibition of osteoblast differentiation and osteoblastic bone formation in skeletal disorders. Microrna214 suppresses osteogenic differentiation of human. In a new study yingxian li and her colleagues show that the microrna mir 214 targets atf4 in osteoblasts to negatively regulate their activity. Sigmaaldrich offers abstracts and fulltext articles by xiaogang wang, baosheng guo, qi li, jiang peng, zhijun yang, aiyuan wang, dong li, zhibo hou, ke lv. R214 targets atf4 to functionally inhibit osteoblast activity in vitro. A transcriptomelevel study identifies changing expression.
The role of micrornas in bone remodeling international journal of. Serum high expression of mir214 and mir5b as novel predictor. An lncrnamrna coexpression network, mirnamrna target prediction network, and. Evidences for a new role of mir214 in chondrogenesis. We prepared cells overexpressing mir 214 and found that mir 214 negatively regulates osteogenic differentiation of hpdlscs. It is targeted by mir214 that inhibits bone formation in mouse. The mir214 inhibits osteoblast activity of activating transcription factor 4 atf4 and regulates the. The role of micrornas in bone remodeling researchgate. Our previous study also identified that mir2143p could target atf4, an important osteogenic transcriptional factor, to suppress bone formation. For the target of mir 214, atf4 protein expression level was decreased after induction.
For the target of mir214, atf4 protein expression level was decreased after. In conclusion, we found that mir 214 atf4 axis is a novel pathway for regulating hpdlsc osteogenic differentiation. Bone remodeling is balanced by bone formation and bone resorption as well as by alterations in the. Micrornas regulate bone development and regeneration. Mirnas target mrnas via complementary basepairing to multiple sites. Microrna214 suppresses osteogenic differentiation of. Moreover, atf4 can also bind to the promoter of rankl and can ultimately inhibit bone resorption.
1213 1570 699 1061 654 1235 949 672 1531 338 1150 1241 1128 34 1506 21 124 127 507 498 1247 1565 1121 756 1359 6 18 1326 17 1410 485 991